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The Microbial Matrix

The “Plant / Soil / Microbial Matrix” is the foundation of all dynamic and sustainable ecosystems. Healthy soils are supported by a vast world of microbes: bacteria and fungi that live in or around the plant system. Ignoring any of the key components will ultimately compromise any regeneration project. Microbes support plant health by increasing the availability of nutrients, enhancing plant root growth, neutralizing toxic compounds in the soil, and making plants more resistant to disease, heat, flooding, and drought. Microbes also deter pathogens and predators that could be harmful to plant establishment and longevity. 

No Biological Treatment                                                                            Treated with RTI AM 120

The photos above show two slide areas along with CA Hwy #1, near Big Sur that were treated with two different re-vegetation protocols. The photo on the left was taken seven years after it had been treated with synthetic fertilizers. The photo of the site on the right was taken one year after it had been treated with organic fertilizer and RTI's AM 120 Mycorrhizal Inoculant.

The Power of Microbes


All members of the animal kingdom, including humans, require billions of microbes within their stomachs to help process foods, protect them from disease, and maintain a healthy biological and metabolic balance. Plants don't have stomachs; however they do have roots, and many of the symbiotic functions that occur in stomachs are replicated on or near the roots of most plants. Exudates are released into the rhizosphere (the area of soil dominated by a plant's root system) which becomes both an environment and food source for a wide range of microbes that form a symbiotic relationship with the host plants.

The Science of Soil Biology

The symbiotic association known as mycorrhizae has been around for over 450 million years, becoming a fundamental support system that enables most plants to survive and thrive in a terrestrial setting. Mycorrhizal fungi make up over 90% of the living microbial biomass that is present in most soils.  The picture provided details mycorrhizal hyphae connecting the fine root hairs to vast regions of the soil, where the hyphae transport carbohydrates to other beneficial microbes, particularly plant growth-promoting Rhizobacteria (PGPR). The mycorrhiza fungus obtains the carbohydrates that it requires from the roots, and in return provides plant nutrients including nitrogen and moisture through the hyphal network. 

The Mycorrhizal / Microbial Matrix   


Mycorrhizal hyphae extend through the entire rhizosphere, the area from which plants can access both moisture and nutrients. A gram of soil may contain around 100 meters of hyphae. The microscopic size of these filaments allows them to penetrate pores and cavities in the soil that much larger roots are unable to access.  While some plant nutrients such as nitrogen are soluble and move freely through the soil, others such as phosphorus remain locked in the soil and must be mined by microbes on behalf of the host plant. Mycorrhizal fungi release phosphates enzymes that can unlock phosphorus and send it back to the plant. PGRPR’s congregate along mycorrhizal hyphae and are responsible for the supply of 11 of the 14 nutrients required for plants. These nutrients include nitrogen, phosphorus, potassium, copper, iron, and zinc.

Helpful mycorrhizal associations are found in over 90 percent of the plants on earth. Here the mycorrhizae create a fine underground network that extends greatly beyond the limits of the tree's roots, greatly increasing their feeding range and actually causing neighboring trees to become physically interconnected.                             

Communities of beneficial bacteria, or PGPR's, congregate along mycorrhizal hyphae where they can transfer elemental nutrients for use by plants in exchange for a food source. A wide range of these PGPR's is responsible for the supply of eleven of the fourteen nutrients required before plants. Ranging from nitrogen to phosphorus and potassium all the way to copper, iron, and zinc. The dynamic interaction between mycorrhizal fungi, plant growth-promoting Rhizobacteria, and their host plants comprise the central components of the “Microbial Matrix.'

Erosion Control

Glomalin, known as "Soil Super Glue" was identified by the USDA / NRCS in 1995. Glomalin is a polysaccharide that is released through mycorrhizal hyphae into the soil. Its primary function appears to be a means by which the soil structure can be modified to enhance the soil texture and stability so a better physical environment is created for aerobic bacteria and fungi. Beneficial microbes that provide nutrients, moisture, and protection to plants prefer an environment with an abundance of air, while pathogens tend to function better in anaerobic environments such as compacted soils. Glomalin also binds coarse sandy soils, reducing the effects of eroding winds and/or water.



Increased access to moisture and nutrients is a direct function of root surface area, which is multiplied by the addition of mycorrhizal hyphae. Plants colonized with mycorrhizal fungi produce significantly larger root masses and are therefore more capable of handling stress from drought, pathogen attacks, and other forms of environmental stress. Mycorrhizal plants have access to more volume, and therefore more moisture compared to plants that are not colonized by mycorrhizal fungi.    

Benefits of Mycorrhizal Inoculation in Restoration Projects

Beginning in 2006, every "World Record Giant Pumpkin", as well as the Canadian, French, British, Dutch, Spanish, and Dutch national records, have been grown with RTI Biologicals. Several state and county records have also had RTI mycorrhizae in their growers' fertilizer programs. The photo to right is the 2022 record US Giant Pumpkin, belonging to Travis Geinger, weighing in at a whopping 2,560 lbs. 

A tablespoon of soil from a healthy ecosystem contains billions of beneficial microbes and enough mycorrhizal hyphae to stretch the length of a football field. In fact, the total biomass of an acre of soil can weigh up to 2,000 lbs, 9 lbs less than this world record giant pumpkin grown with mycorrhizal inoculum from RTI. Of all this total biomass, mycorrhizal fungi in the form of hyphae, spores, and colonized roots will comprise 90% of the total weight and mass!


A soil without microbes is not complete, and can not become a self-sustaining ecosystem. With a symbiotic relationship that dates back 450 million years, mycorrhizal associations have evolved into a critical component of successful restoration projects across several continents. Since 1993, RTI has been the leading technology innovator in the development of biological nutritional products for forestry, habitat restoration, agriculture, landscape management, and home gardening. 

Click on the PDF to read more about our mycorrhizal fungi in habitat restoration projects.

Visit to learn more about our research and field trials.
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